Published in

American Society for Microbiology, Journal of Virology, 19(85), p. 10415-10420, 2011

DOI: 10.1128/jvi.05071-11

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Construction of a Lytically Replicating Kaposi's Sarcoma-Associated Herpesvirus

Journal article published in 2011 by Matthias Budt, Tsvetana Hristozova, Georg Hille ORCID, Katrin Berger, Wolfram Brune ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Karposi's sarcoma-associated herpesvirus (KSHV) is found predominantly in a latent state in most cell types, impeding investigations of the lytic replication cycle. Here, we engineered the cloned KSHV genome, bacterial artificial chromosome 36 (BAC36), to enforce constitutive expression of the main lytic switch regulator, the replication and transcription activator (RTA) (open reading frame 50 [ORF50]). The resulting virus, KSHV-lyt, activated by default the lytic cycle and replicated to high titers in various cells. Using KSHV-lyt, we showed that ORF33 (encoding a tegument protein) is essential for lytic KSHV replication in cell culture, but ORF73 (encoding the latent nuclear antigen [LANA]) is not. Thus, KSHV-lyt should be highly useful to study viral gene function during lytic replication.