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Wiley Open Access, Journal of Cellular and Molecular Medicine, 1(11), p. 139-147, 2007

DOI: 10.1111/j.1582-4934.2007.00006.x

Wiley Open Access, Journal of Cellular and Molecular Medicine, 4(10), p. 1-9, 2006

DOI: 10.1111/j.1582-4934.2006.tb00525.x

Wiley Open Access, Journal of Cellular and Molecular Medicine, 4(10), p. 1-9

DOI: 10.1111/j.1582-4934.2006.tb00446.x

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Inhibition of adhesion molecule expression on human venous endothelial cells by non-viral siRNA transfection

Journal article published in 2006 by Tobias Walker, Hans P. Wendel, Liane Tetzloff, Claudia Raabe, Olaf Heidenreich ORCID, Perikles Simon ORCID, Albertus M. Scheule, Gerhard Ziemer
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Objective: Expression of adhesion molecule receptors on venous endothelial cells crucially influences the fate of venous grafts by mediating leukocyte-endothelium interactions. These interactions include adhesion of leukocytes to the endothelium, followed by transendothelial migration, leading to neointimal hyperplasia and finally graft occlusion. Therefore, inhibition of adhesion molecule expression may be a promising strategy to improve the quality of venous grafts. We tested the efficiency of non-viral transfection of human venous endothelial cells with short interfering RNA (siRNA) to specificially down-regulate adhesion molecule expression. Methods: Primary cultures of human venous endothelial cells (HVEC) were examined for expression of the adhesion molecules ICAM1, VCAM1 and E-selectin (SELE) after non viral siRNA transfection. Adhesion molecule expression was measured by flow cytometry, real-time PCR and immunoblotting after stimulation with TNF-alpha, an inflammatory cytokine. Results: Non-transfected cells showed a strong increase of adhesion molecule expression following cytokine stimulation (p