: The spatiotemporal features of walking in toddlers are known to be related to the level of maturation of the central nervous system. However, previous studies did not assess whether there could be an effect of clothes on the acquisition of walking. In this study, it was hypothesized that clothes modify the toddlers' walking. To test this hypothesis, 22 healthy toddlers divided into 3 groups of walking experience were assessed in four clothing conditions (Diaper+Trousers; Diaper+Pants of tracksuit; Diaper; Underwear). Results revealed significant effects of clothing on velocity and step length of toddlers from 6 to 18 months of walking experience. These results suggested that biomechanical constraints induced by the textile features alter the walking of toddlers. Therefore, in studies of toddler's gait, the clothing worn should be carefully mentioned and controlled.