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American Society for Microbiology, Applied and Environmental Microbiology, 5(74), p. 1649-1652, 2008

DOI: 10.1128/aem.01864-07

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Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening▿ †

Journal article published in 2008 by Jonathan E. Schmitz, Anu Daniel, Mattias Collin, Raymond Schuch, Vincent A. Fischetti
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli . As proof of principle, several anti- Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.