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American Society for Microbiology, Applied and Environmental Microbiology, 4(74), p. 1223-1231, 2008

DOI: 10.1128/aem.01726-07

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Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii

Journal article published in 2007 by Nr R. Mullane, M. Ryan ORCID, C. Iversen, M. Murphy, P. O’Gaora, T. Quinn, P. Whyte, P. G. Wall, C. Fanning, S. Fanning
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii . The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.