Published in

Optica, Biomedical Optics Express, 7(3), p. 1594, 2012

DOI: 10.1364/boe.3.001594

Links

Tools

Export citation

Search in Google Scholar

Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

Journal article published in 2012 by Keisuke Isobe, Hiroyuki Kawano, Takanori Takeda, Akira Suda, Akiko Kumagai, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Red circle
Preprint: archiving forbidden
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

We demonstrate how the resolution and imaging depth limitations of nonlinear optical microscopy can be overcome by modulating the spatial overlap between two-color pulses. We suppress out-of-focus signals, which limit the imaging depth, by a factor of 100, and enhance the lateral and axial resolution by factors of 1.6 and 1.4–1.8 respectively. Using spatial overlap modulation, we demonstrate background-free three-dimensional imaging of fixed mouse brain tissue at depths for which the signals of the conventional technique are swamped by background noise from out-of-focus regions.