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Wiley Open Access, Clinical and Translational Allergy, S1(1), 2011

DOI: 10.1186/2045-7022-1-s1-p4

Croatian Chemical Society, Croatica Chemica Acta, 3(84), p. 419-422

DOI: 10.5562/cca1790

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Expression, purification and crystallization of wheat profilin (Tri a 12)

Journal article published in 2011 by Peter Forstenlechner, Margit Cichna-Markl, Christina Ecker, Karin Hoffmann-Sommergruber ORCID, Dalibor Milić, Dubravka Matković-Čalogović
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees. (doi: 10.5562/cca1790)