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American Association for the Advancement of Science, Science, 6109(338), p. 932-936, 2012

DOI: 10.1126/science.1225624

Elsevier, Biophysical Journal, 2(104), p. 545a, 2013

DOI: 10.1016/j.bpj.2012.11.3022

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Synthetic lipid membrane channels formed by designed DNA nanostructures

Journal article published in 2012 by Martin Langecker, Vera Arnaut, Thomas G. Martin, Jonathan List, Stephan Renner, Michael Mayer ORCID, Hendrik Dietz, Friedrich C. Simmel
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We created nanometer-scale transmembrane channels in lipid bilayers using self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrates and spans a lipid membrane, and a barrel-shaped cap that adheres to the membrane in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we find similarities to the response of natural ion channels, such as conductances on the order of 1 nS and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. In single-molecule translocation experiments, we highlight one of many potential applications of the synthetic channels, namely as single DNA molecule sensing devices.