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Oxford University Press (OUP), The Journal of Infectious Diseases, 2(200), p. 273-278

DOI: 10.1086/599987

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Esterase D Is Essential for Protection ofNeisseria gonorrhoeaeagainst Nitrosative Stress and for Bacterial Growth during Interaction with Cervical Epithelial Cells

Journal article published in 2009 by Adam J Potter, Stephen P Kidd ORCID, Jennifer L Edwards, Megan L Falsetta, Michael A Apicella, Michael P Jennings, Alastair G McEwan
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

estD encodes a carboxylic ester hydrolase and is part of the NmlR regulon in Neisseria gonorrhoeae. An estD mutant was found to be susceptible to nitrite and to S-nitrosoglutathione. This mutant was also unable to infect and survive within human cervical epithelial cells, and it showed reduced ability to form a biofilm on these cells. We conclude that esterase D is an integral part of the nitrosative stress defense system of N. gonorrhoeae and that it has potential importance in pathogenesis. Neisseria gonorrhoeae is an obligate human pathogen. Gono-coccal infection is associated with inflamed mucosa of the ure-thra in males but often results in asymptomatic infection of the cervix in females [1]. The innate immune response produces reactive oxygen species and reactive nitrogen species (RNS), and some of the strategies used by N. gonorrhoeae against these host defense molecules have been reviewed elsewhere [2]. De-fense against RNS may be of particular importance; gonococci