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BioMed Central, Genome Biology, 8(14), p. R94

DOI: 10.1186/gb-2013-14-8-r94

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Measuring cell-type specific differential methylation in human brain tissue

Journal article published in 2013 by Carolina M. Montaño, Rafael A. Irizarry, Walter E. Kaufmann, Konrad Talbot, Raquel E. Gur, Andrew P. Feinberg, Margaret A. Taub
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The behavior of epigenetic mechanisms in the brain is obscured by tissue heterogeneity and disease-related histological changes. Not accounting for these confounders leads to biased results. We develop a statistical methodology that estimates and adjusts for celltype composition by decomposing neuronal and non-neuronal differential signal. This method provides a conceptual framework for deconvolving heterogeneous epigenetic data from postmortem brain studies. We apply it to find cell-specific differentially methylated regions between prefrontal cortex and hippocampus. We demonstrate the utility of the method on both Infinium 450k and CHARM data.