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Elsevier, Carbohydrate Polymers, (133), p. 539-546

DOI: 10.1016/j.carbpol.2015.07.060

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(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity

Journal article published in 2015 by Kassandra S. M. Oliveira, Mirela Di Bastiani, Lucimara M. C. Cordeiro ORCID, Mírian F. Costa, Karina A. Toledo, Marcello Iacomini, Aneli M. Babosa, Robert F. H. Dekker, Valéria M. G. Nascimento
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This paper was not found in any repository, but could be made available legally by the author.

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Abstract

The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern. ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)