Two new polyhydroxypregnane glycosides, namely cynotophyllosides G-H (1-2), were isolated from the roots of Cynanchum otophyllum, together with three known steroids. Their structures were established by spectroscopic methods and acid-catalysed hydrolysis. Cynanchum otophyllum also known as “ Qingyangshen“ in traditional Chinese medicine, is a perennial weed which has been used locally to treat epilepsy, rheumatic pain, kidney weakness, and muscle injuries.