The effect of forward and reverse torsion on flow behavior and microstructure evolution, particularly dynamic and static spheroidization, on Ti-6Al-4V with an alpha lamella colony microstructure was studied. Testing was undertaken sub beta transus [1088 K (815 °C)] at strain rates of either 0.05 or 0.5 s−1. Quantitative metallography and electron back scatter diffraction has identified that a critical monotonic strain (εc) in the range of 0.3 to 0.6 is required to initiate rapid dynamic spheroidization of the alpha lamella. For material deformed to strains below εc and then reversed to a zero net strain the orientation relationships between alpha colonies are close to ideal Burgers, enabling prior beta grains to be fully reconstructed. Material deformed to strains greater than εc and reversed lose Burgers and no beta reconstruction is possible, suggesting εc is the strain required to generate break-up of lamella. Static spheroidization is, however, sensitive to strain path around εc. Annealing at 1088 K (815 °C) for 4 hours for material subjected to 0.25 forward + 0.25 forward strain produces 48 pct spheroidized grains while material with 0.25 forward + 0.25 reverse strain has 10 pct spheroidization. This is believed to be a direct consequence of different levels of the stored energy between these two strain paths.