Published in

MDPI, Viruses, 1(5), p. 150-161, 2013

DOI: 10.3390/v5010150

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Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms

Journal article published in 2013 by Pieter Moons ORCID, David Faster, Abram Aertsen
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations.