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BioMed Central, Genome Biology, 11(14), p. R124

DOI: 10.1186/gb-2013-14-11-r124

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AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation

Journal article published in 2013 by Sarah Aldridge, Stephen Watt, Michael A. Quail, Tim Rayner ORCID, Margus Lukk, Michael F. Bimson, Daniel Gaffney, Duncan T. Odom ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days.