American Society for Microbiology, Infection and Immunity, 5(61), p. 2200-2202, 1993
DOI: 10.1128/iai.61.5.2200-2202.1993
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A mutant of diphtheria toxin in which Ile-364 was replaced by Lys was at least 500-fold less toxic to Vero cells than the parental toxin. Its ability to undergo low-pH-triggered translocation across the plasma membrane was greatly diminished, as was its ability to form ion-conductive channels. In addition, the mutant toxin was inactive in the pH-dependent killing of Escherichia coli.