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Nature Research, Nature Methods, 8(10), p. 759-761, 2013

DOI: 10.1038/nmeth.2533

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Engineered Nanostructured β-Sheet Peptides Protect Membrane Proteins

Journal article published in 2013 by Houchao Tao, Sung Chang Lee, Arne Moeller, Rituparna Sinha Roy, Fai Yiu Siu, Jörg Zimmermann, Raymond C. Stevens, Clinton S. Potter, Bridget Carragher ORCID, Qinghai Zhang
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.