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Wiley, Chemistry - A European Journal, 25(16), p. 7596-7604, 2010

DOI: 10.1002/chem.201000487

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Activity and Enantioselectivity of the Hydroxynitrile Lyase MeHNL in Dry Organic Solvents

Journal article published in 2010 by Monica Paravidino, Menno J. Sorgedrager, Ulf Hanefeld, Romano V. A. Orru ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross-linked enzyme aggregate (MeHNL-CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity.