ABSTRACTWe used atomic force microscope (AFM) to acquire high-resolution images of collagen type I triple-helices under ambient conditions in tapping mode. Angles between consecutive fixed-length segments were measured and analyzed to yield persistence length and elastic constant. Changing the segment length allowed exploring the mechanics at various scales. Understanding the mechanical properties of collagen molecules could serve to elucidate mechanisms of complex mechanical properties of interest in nanomedicine and nanotechnology.