Elsevier, Cancer Genetics and Cytogenetics, 2(199), p. 101-109, 2010
DOI: 10.1016/j.cancergencyto.2010.02.009
Elsevier, Cancer Genetics and Cytogenetics, 2(202), p. 71-75
DOI: 10.1016/j.cancergencyto.2010.06.009
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When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used: small nuclear RNAs and small nucleolar RNAs. In this study, six different endogenous control genes for miRNA studies were investigated in prostate tissue material from the Swedish Watchful Waiting cohort. The stability of the controls was investigated using two different software applications, NormFinder and BestKeeper. RNU24 was the most suitable endogenous control gene for miRNA studies in prostate tissue materials. ; Ingår i projekt1? Ingår i projekt Om publikationen ingår i ett projekt, ange projektets namn. För att ange flera projekt, klicka på Ytterligare projekt. x