Published in

American Society for Microbiology, Molecular and Cellular Biology, 4(22), p. 1246-1252, 2002

DOI: 10.1128/mcb.22.4.1246-1252.2002

Links

Tools

Export citation

Search in Google Scholar

Convergence of TOR-Nitrogen and Snf1-Glucose Signaling Pathways onto Gln3

Journal article published in 2002 by Paula G. Bertram, Jae H. Choi, John Carvalho, Ting-Fung Chan ORCID, Wandong Ai, X. F. Steven Zheng
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Carbon and nitrogen are two basic nutrient sources for cellular organisms. They supply precursors for energy metabolism and metabolic biosynthesis. In the yeast Saccharomyces cerevisiae, distinct sensing and signaling pathways have been described that regulate gene expression in response to the quality of carbon and nitrogen sources, respectively. Gln3 is a GATA-type transcription factor of nitrogen catabolite-repressible (NCR) genes. Previous observations indicate that the quality of nitrogen sources controls the phosphorylation and cytoplasmic retention of Gln3 via the target of rapamycin (TOR) protein. In this study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor. Our data show that glucose and nitrogen signaling pathways converge onto Gln3, which may be critical for both nutrient sensing and starvation responses.