A new core–shell nanoparticle containing the chemotherapeutic drug doxorubicin was formulated via amphiphilic graft copolymer self-assembly using curdlan- graft-poly(ethylene glycol) (curdlan -g-PEG). The graft copolymer was synthesized through the dicyclohexylcarbodiimide ester linkage of carboxylated PEG to the hydroxyl groups of the curdlan backbone. The nanoparticles were 109.9 nm in size and encapsulated doxorubicin in high yield (4%–5% wt/wt). The nanoparticles also controlled the release of doxorubicin over 24 h with a release profile that followed a Fickian diffusion model. The biocompatibility of curdlan- g-PEG was confirmed by hemolysis assay. This is the first nanoparticle formulated using the hydrophobicity of curdlan for concealing the immunomodulatory potential of curdlan within the core.