Published in
National Academy of Sciences, Proceedings of the National Academy of Sciences, 34(109), p. 13853-13858, 2012
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SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
,
Nathalie Gonzalez,
Jelle Van Leene,
Katrien Maleux,
Hannes Vanhaeren,
Liesbeth De Milde,
Stijn Dhondt,
Leen Vercruysse,
Raphaël Mercier,
Van Jelle Leene,
Erwin J. Witters,
Laurence Cromer,
Gerrit Ts S. Beemster ,
Han K. Remaut,
Marc C. E. Van Montagu
and other authors.
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Abstract
The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana , SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.