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National Academy of Sciences, Proceedings of the National Academy of Sciences, 1(106), p. 79-84, 2009

DOI: 10.1073/pnas.0811468106

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Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays

Journal article published in 2009 by Walter Reisner, Niels B. Larsen ORCID, Henrik Flyvbjerg, Jonas O. Tegenfeldt, Anders Kristensen
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into “connect-the-dots” conformations consisting of locally pinned segments joined by fluctuating linkers. These results have broad implications for nanotechnology fields that require methods for the nanoscale positioning and manipulation of DNA.