<b><i>Introduction:</i></b> The relationship between ejaculatory frequency and ejaculatory latency time is under-investigated. <b><i>Subjects and Methods:</i></b> 120 healthy subjects were enrolled in a prospective randomized trial. Patients in group A engaged in sexual intercourse after a 10-day abstinence period for 3 months. Patients in group B engaged in sexual intercourse twice a week for 3 months. Ejaculation function was evaluated through the measurement of the intravaginal ejaculatory latency time (IELT) and through the premature ejaculation diagnostic tool (PEDT) at baseline and at the end of the study. <b><i>Results:</i></b> At the end of the study, mean IELT values in group A were significantly lower compared to baseline values. Results from the PEDT questionnaire at the end of the study demonstrated a significantly worse ejaculatory control and ability to delay ejaculation by patients in group A. <b><i>Conclusions:</i></b> Reduced ejaculatory frequency impairs ejaculation control.