<b><i>Objective:</i></b> The objective of this survey was to assess oral hygiene habits and<b> </b>compliance with guidelines for good oral health set forth by the Italian Ministry of Health (IMH). <b><i>Subjects and Methods:</i></b> A sample of 2,200 self-administered questionnaires was sent to four dental clinics across Italy to assess sociodemographic information, oral hygiene habits, frequency of dental visits and services received at previous visits among a population of adult patients. <b><i>Results:</i></b> Of the 2,200 questionnaires, 1,201 (54.6%) were returned. Findings showed that full compliance with the IMH recommendations was low (12%): a small number of patients (n = 223, 18.6%) visited a dentist every 6 months and only 256 (23.5%) brushed their teeth at least twice a day. <b><i>Conclusion:</i></b> Our data showed that regular attendance (at least 1 visit/year) at dental clinics for routine check-up and brushing teeth at least twice a day were poor. Therefore, we recommend that clinicians educate and motivate their patients about the benefits of healthy oral hygiene practices.