Hyalomma scupense is a two-host tick infesting mainly cattle representing in North Africa the vector of tropical theileriosis (Theileria annulata infection), a major tick-borne disease affecting cattle. Any effective control programme of ticks requires a good knowledge of the biology of the target species. In the present study, three cattle farms in northeast Tunisia were surveyed during the activity seasons for adult and nymphs of Hyalomma scupense. Several indicators were studied, including chronological indicators, infestation prevalence, infestation intensity and feeding predilection sites of the ticks. The adult ticks were present from mid-June to late November. Nymphs were observed on animals from early September to late November. A large proportion of the ticks were attached in the posterior udder quarters: 41% and 64% of adult ticks and nymphs, respectively. The animals that were heavily infested by adult ticks were also heavily infested by nymphs. Moreover, 17% of adult ticks and 53% of nymphs were present on only 5% of cattle population. These data are important for the success of targeted acaricide application leading to a dramatic decrease of acaricide quantity needed for the treatment. When the preferential sites of attachment are known, the effectiveness of manual removal of ticks can be improved. The presence of highly infested animals is to be considered when any control programme is implemented, since these animals harbour a high proportion of the ticks.