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Karger Publishers, Cytogenetic and Genome Research, 1-2(88), p. 114-118, 2000

DOI: 10.1159/000015502

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FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements

Journal article published in 2000 by J. Weimer, M. Kiechle, N. Arnold ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints.