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American Society for Microbiology, Applied and Environmental Microbiology, 3(79), p. 1028-1033, 2013

DOI: 10.1128/aem.02644-12

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Recruitment and Rearrangement of Three Different Genetic Determinants into a Conjugative Plasmid Increase Copper Resistance in Pseudomonas syringae

Journal article published in 2012 by José A. Gutiérrez-Barranquero, Antonio de Vicente ORCID, Víctor J. Carrión, George W. Sundin, Francisco M. Cazorla
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae . Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper.