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Hindawi, Journal of Nucleic Acids, (2013), p. 1-7, 2013

DOI: 10.1155/2013/629218

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R-Loop FormationIn Transat an AGGAG Repeat

Journal article published in 2013 by Kazuya Toriumi ORCID, Takuma Tsukahara, Ryo Hanai ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.