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BioMed Central, Arthritis Research and Therapy, 6(13), p. R208

DOI: 10.1186/ar3541

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Frequency of Th17 CD20+ cells in the peripheral blood of rheumatoid arthritis patients is higher compared to healthy subjects

Journal article published in 2011 by Edwin Bremer, Paul Bremer E. Tarr J. M. de Bruyn M. Helfrich W. Kendall A. C. Haigh R. C. Viner N. J. and Winyard P. G. Eggleton, Joanna M. Tarr, Marco de Bruyn ORCID, Wijnand Helfrich ORCID, Alexandra Kendall, Richard C. Haigh, Nick J. Viner, Paul G. Winyard
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract Introduction Rheumatoid arthritis (RA) is considered a T cell driven autoimmune disease, therefore, the ability of B cell depleting biologics, e.g., anti-CD20 antibodies, to alleviate RA is unclear. This study examined the proportions of IL-17-secreting lymphocytes in the blood of healthy subjects and RA patients and determined if Th17 cells belong to a CD20+ subset of T cells. Methods Fluorescence-activated cell sorting and confocal microscopy verified CD3, CD4/CD8 and CD20-staining of T cells. IL-17 secretion was determined using a commercial assay. Results In healthy subjects and RA patients blood, the median percentage of total CD20+ lymphocytes was similar (7.5%; n = 6 and 10.3%; n = 9, respectively) and comprised predominantly of B cells (~ 86%). However, 2-4% of CD3+ T cells from both healthy subjects ( n = 7) and RA ( n = 8) individuals co-expressed CD20. The peripheral blood of healthy subjects contained few IL-17-secreting CD20+ T cells (