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American Society for Microbiology, Microbiology and Molecular Biology Reviews, 1(73), p. 62-70, 2009

DOI: 10.1128/mmbr.00028-08

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Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level

Journal article published in 2009 by Martin Keller, Robert Hettich ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

SUMMARY The increase in sequencing capacity led to a new wave of metagenomic projects, enabling and setting the prerequisite for the application of environmental proteomics technologies. This review describes the current status of environmental proteomics. It describes sample preparation as well as the two major technologies applied within this field: two-dimensional electrophoresis-based environmental proteomics and liquid chromatography-mass spectrometry-based environmental proteomics. It also highlights current publications and describes major scientific findings. The review closes with a discussion of critical improvements in the area of integrating experimental mass spectrometry technologies with bioinformatics as well as improved sample handling.