Nature Research, Nature Cell Biology, 8(13), p. 914-923, 2011
DOI: 10.1038/ncb2281
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Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding protein Wntless (Wls), which transports Wnt from the Golgi network to the cell surface for release. It has recently been shown that recycling of Wls through a retromer-dependent endosome-to-Golgi trafficking pathway is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is poorly understood. Here, we report that Wls recycling is mediated through a retromer pathway that is independent of the retromer sorting nexins SNX1-SNX2 and SNX5-SNX6. We have found that the unrelated sorting nexin, SNX3, has an evolutionarily conserved function in Wls recycling and Wnt secretion and show that SNX3 interacts directly with the cargo-selective subcomplex of the retromer to sort Wls into a morphologically distinct retrieval pathway. These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo. [KEYWORDS: Animals, Animals, Genetically Modified, Biological Transport, Active, Caenorhabditis elegans/genetics/growth & development/metabolism, Drosophila/genetics/growth & development/metabolism, Endosomes/metabolism, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins/ metabolism, Models, Biological, RNA Interference, Signal Transduction, Sorting Nexins/antagonists & inhibitors/genetics/ metabolism, Wnt Proteins/ metabolism, trans-Golgi Network/metabolism] ; Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding protein Wntless (Wls), which transports Wnt from the Golgi network to the cell surface for release. It has recently been shown that recycling of Wls through a retromer-dependent endosome-to-Golgi trafficking pathway is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is poorly understood. Here, we report that Wls recycling is mediated through a retromer pathway that is independent of the retromer sorting nexins SNX1-SNX2 and SNX5-SNX6. We have found that the unrelated sorting nexin, SNX3, has an evolutionarily conserved function in Wls recycling and Wnt secretion and show that SNX3 interacts directly with the cargo-selective subcomplex of the retromer to sort Wls into a morphologically distinct retrieval pathway. These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo. [KEYWORDS: Animals, Animals, Genetically Modified, Biological Transport, Active, Caenorhabditis elegans/genetics/growth & development/metabolism, Drosophila/genetics/growth & development/metabolism, Endosomes/metabolism, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins/ metabolism, Models, Biological, RNA Interference, Signal Transduction, Sorting Nexins/antagonists & inhibitors/genetics/ metabolism, Wnt Proteins/ metabolism, trans-Golgi Network/metabolism]