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BioMed Central, Biology Direct, 1(8), 2013

DOI: 10.1186/1745-6150-8-6

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Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

Journal article published in 2013 by Guilherme Loss-Morais, Guilherme Loss de Morais, Peter M. Waterhouse ORCID, Rogerio Margis
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.