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Wiley, Angewandte Chemie International Edition, 33(51), p. 8343-8347, 2012

DOI: 10.1002/anie.201202094

Wiley, Angewandte Chemie, 33(124), p. 8468-8472, 2012

DOI: 10.1002/ange.201202094

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GRecon: A Method for the Lipid Reconstitution of Membrane Proteins**

Journal article published in 2012 by Thorsten Althoff ORCID, Karen M. Davies, Sabrina Schulze, Friederike Joos, Werner Kühlbrandt
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Density gradients with increasing concentrations of cyclodextrin and detergent-destabilized liposomes are loaded with detergent-solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle method separates proteoliposomes from empty liposomes and is particularly suitable for the lipid reconstitution of large, fragile membrane proteins.