The effects of the nitrification inhibitors nitrapyrin, acetylene (provided by wax-coated calcium carbide), and phenylacetylene on nitrogen (N) transformations and denitrification losses following presowing applications of urea were determined in a cottonfield in the Namoi Valley of New South Wales. The study used 0.05-m-diameter microplots to follow the changes in mineral N, and 0.15-m-diameter microplots fertilised with 15N-labelled urea (6 g N/ m2; 5 atom % 15N) to assess losses of applied N. When urea was applied in February (34 weeks before sowing), 84% of applied N was lost from the soil. Loss of applied N was reduced by addition of nitrapyrin and phenylacetylene, to 53 and 57%, respectively. In the absence of nitrification inhibitors, less N was lost (72% of that applied) from an application in May than from the February application. Addition of acetylene, phenylacetylene, and nitrapyrin reduced losses over the 24 weeks to sowing to 57, 52, and 48%, respectively. These experiments show that N loss from presowing applications of urea can be significantly reduced by the use of nitrification inhibitors, but that the losses of N are still substantial.