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Taylor and Francis Group, Plant Signaling & Behavior, 12(5), p. 1547-1548, 2010

DOI: 10.4161/psb.5.12.13994

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Efficient procedure for site-directed mutagenesis mediated by PCR insertion of a novel restriction site

Journal article published in 2010 by Hatem Rouached ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Better understanding of proteins' structure/function relationship and dissecting their functional domains are still challenges yet to be mastered. Site-directed mutagenesis approaches that can alter bases at precise positions on the gene sequence can help to reach this goal. This article describes an efficient strategy that can be applied not only for both deletion and substitution of target amino acids, but also for insertion of point mutations in promoter regions to study cis-regulating elements. This method takes advantage of the plasticity of the genetic code and the use of compatible restriction sites.