Published in
Ivyspring International Publisher, Journal of Genomics, (3), p. 36-39
DOI: 10.7150/jgen.9204
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- Ivyspring International Publisher | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net]
- [www.ncbi.nlm.nih.gov] | PDF
Tools
Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS
Journal article published in 2015 by
Giovanni Bacci,
Alessia Bani,
Marco Bazzicalupo,
Maria Teresa Ceccherini,
Marco Galardini 
,
Paolo Nannipieri,
Giacomo Pietramellara,
Alessio Mengoni
,
Paolo Nannipieri,
Giacomo Pietramellara,
Alessio Mengoni
This paper is made freely available by the publisher.
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Abstract
Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignments.