Cold Spring Harbor Protocols, 6(2009), p. pdb.prot5227-pdb.prot5227
DOI: 10.1101/pdb.prot5227
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INTRODUCTIONThis protocol describes the preparation and staining of honeybee embryos for immunohistochemistry. An antigen retrieval step is required in order to restore antigens that have become masked by the long fixation step required for honeybee embryos.