Published in

Cold Spring Harbor Protocols, 6(2009), p. pdb.prot5226-pdb.prot5226

DOI: 10.1101/pdb.prot5226

Links

Tools

Export citation

Search in Google Scholar

In Situ Hybridization of Sectioned Honeybee (Apis mellifera) Tissues: Figure 1.

Journal article published in 2009 by Peter K. Dearden, Elizabeth J. Duncan ORCID, Megan J. Wilson
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

Full text: Unavailable

Green circle
Preprint: archiving allowed
Upload
Red circle
Postprint: archiving forbidden
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

INTRODUCTIONThis protocol outlines how to carry out section in situ hybridization on honeybee larvae to detect expression of a gene of interest in individual cell types. It can also be modified to investigate gene expression in a sectioned honeybee brain. A digoxigenin-labeled probe is hybridized to fixed sections and the probe is detected by 4-nitro blue tetrazolium chloride (NBT)-5-bromo-4-chloro-3-indolyl-phosphate (BCIP) color development.