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Cold Spring Harbor Protocols, 6(2009), p. pdb.prot5225-pdb.prot5225

DOI: 10.1101/pdb.prot5225

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Whole-Mount In Situ Hybridization of Honeybee (Apis mellifera) Tissues

Journal article published in 2009 by Peter K. Dearden, Elizabeth J. Duncan ORCID, Megan J. Wilson
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

INTRODUCTIONThis protocol outlines how to perform in situ hybridization on honeybee embryos, adult brains, or ovaries to detect the expression pattern of a targeted transcript. The tissue is digested by proteinase K to promote probe penetration, and then prehybridized to block nonspecific binding of the probe.