American Chemical Society, Journal of Medicinal Chemistry, 9(48), p. 3364-3371, 2005
DOI: 10.1021/jm0500471
Full text: Unavailable
The compounds [Pt(Me2phen)(acy)2](NO3)2 (1), [Pt(Me2phen)(pen)2](NO3)2, [Pt(phen)(acy)2](NO3)2 (2), and [Pt(phen)(pen)2](NO3)2, containing the bidentate 1,10-phenanthroline (phen) or 2,9- dimethyl-1,10-phenanthroline (Me2phen, neocuproine) and the antiviral agents acyclovir (acy) or penciclovir (pen), show different in vitro toxicity, the Me2phen complexes being appreciably more toxic than the phen complexes. To explain the different behavior, we investigated the reaction of complexes 1 and 2 with glutathione (ç-glutamylcysteinylglycine, GSH), a peptide believed to play an important role in driving the cellular effects of platinum drugs. The reaction led to different products, the phen complexes forming a stable binuclear í-thiol-bridged species still containing the phenanthroline and the Me2phen complexes releasing the neocuproine ligand and forming an insoluble material. In vitro tests confirmed that the greater cell toxicity of complex 1 is due to the displacement of the neocuproine ligand by GSH. The results highlight the great dependence of the glutathione reactivity upon relatively small changes in the platinum coordination sphere.