Published in

MyJove Corporation, Journal of Visualized Experiments, 75, 2013

DOI: 10.3791/3899-v

MyJove Corporation, Journal of Visualized Experiments, 75, 2013

DOI: 10.3791/3899

Links

Tools

Export citation

Search in Google Scholar

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Journal article published in 2013 by Lisa Zeigler Allen, Lisa Zeigler Allen, Thomas Ishoey, Mark A. Novotny, Jeffrey S. McLean ORCID, Roger S. Lasken, Shannon J. Williamson
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Red circle
Preprint: archiving forbidden
Orange circle
Postprint: archiving restricted
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Whole genome amplification and sequencing of single microbial cells enables genomic characterization without the need of cultivation (1-3). Viruses, which are ubiquitous and the most numerous entities on our planet (4) and important in all environments (5), have yet to be revealed via similar approaches. Here we describe an approach for isolating and characterizing the genomes of single virions called 'Single Virus Genomics' (SVG). SVG utilizes flow cytometry to isolate individual viruses and whole genome amplification to obtain high molecular weight genomic DNA (gDNA) that can be used in subsequent sequencing reactions.