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American Society for Microbiology, Molecular and Cellular Biology, 1(10), p. 387-390, 1990

DOI: 10.1128/mcb.10.1.387-390.1990

American Society for Microbiology, Molecular and Cellular Biology, 1(10), p. 387-390, 1990

DOI: 10.1128/mcb.10.1.387

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Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site.

Journal article published in 1990 by Rosario Ammendola, De Simone, Fotini Gounari, Giulia Piaggio ORCID, Vincenzo De Simone, Riccardo Cortese
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.