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American Society for Microbiology, Journal of Bacteriology, 18(178), p. 5537-5539, 1996

DOI: 10.1128/jb.178.18.5537-5539.1996

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In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).

Journal article published in 1996 by Madalena Salema ORCID, Inácia Capucho, Bert Poolman, M. V. San Romão, M. C. Dias
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M. D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.