Published in

Wiley, ChemBioChem, 5(16), p. 752-755, 2015

DOI: 10.1002/cbic.201500013

Links

Tools

Export citation

Search in Google Scholar

Formation and Abundance of 5-Hydroxymethylcytosine in RNA

Journal article published in 2015 by Sabrina M. Huber, Pieter van Delft, Lee Mendil, Martin Bachman, Katherine Smollett, Finn Werner, Eric A. Miska ORCID, Shankar Balasubramanian
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Furthermore, we have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells.