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解磷真菌C2'的分离鉴定及其在土壤中实际解磷效果的研究

Published in 2009 by 王莉晶;高晓蓉;吕军;安利佳
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

以不溶性磷酸钙为筛选培养基,从墨西哥万年草根际土壤中分离筛选出一株高效解磷真菌C2'。经过形态观察和核糖体间隔区ITS扩增序列分析,确定该菌株为草酸青霉菌属(Penicillium oxalicum)。研究了不同碳源、氮源及盐浓度对其解磷能力的影响。利用高效液相色谱法(HPLC)对C2'的解磷机理进行初步研究,结果发现代谢过程中产生的苹果酸和酒石酸可能对溶磷起重要作用。土壤中实际的解磷效果表明,C2'对小麦苗期的生长有明显的促进作用。 ; 辽宁省基金资助项目(20062182)资助