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Elsevier, MethodsX, (2), p. 141-144, 2015

DOI: 10.1016/j.mex.2015.03.004

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Label-free method for anti-glucopeptide antibody detection in Multiple Sclerosis

Journal article published in 2015 by Feliciana Real Fernández, Feliciana Real-Fernández, Giada Rossi, Francesco Lolli, Anna Maria Papini, Paolo Rovero ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Surface plasmon resonance technique is particularly interesting in immunology because it has the potential to visualize label-free antigen-antibody interactions in real-time, thus enabling antibody detection and monitoring. Herein we release the guidelines for the correct use of a method to detect specific antibodies directly in Multiple Sclerosis patients’ sera using a glucopeptide-based label-free biosensor. The protocol describes the strategy employed for the immobilization of glucopeptide antigen onto a gold sensor chip and the evaluation of the specific binding of serum antibodies to the immobilized antigen.