Published in
Oxford University Press, Nucleic Acids Research, 23(31), p. 6758-6769
DOI: 10.1093/nar/gkg911
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Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside
,
Koen Nauwelaerts,
Veerle Kempeneers,
Helmut Rosemeyer,
Arthur Van Aerschot,
Roger Busson,
Jeffrey C. Lacey,
Lacey Jc,
Ekaterina Efimtseva,
Sergey Mikhailov ,
Eveline Lescrinier ,
Piet Herdewijn
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Abstract
A ribose residue inserted between the 3′-OH of one nucleotide and the 5′-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.