Biological traits are increasingly used for describing ecological functioning of stream benthic assem- blages. Such approaches associate information on species distribution to their biological characteristics (e.g. life history, physiology, dispersal ability) providing a biological trait profile of communities. They may complement structural bioassessment measures using taxonomic composition by providing indirect information on stream ecological functioning, with the additional advantage of being less constrained by biogeographic differences. A multivariate predictive model, that provides a site-specific list of expected taxa at least disturbed conditions was recently developed for the bioassessment of Portuguese streams. Here, we tested if the inclusion of trait information in the model would also enable the detection of most common anthropogenic disturbances (i.e., organic contamination, hydrological disturbance) and provide diagnostic hints for causal relationships. We used existing information on 11 invertebrate biological traits and their 54 categories to convert the observed and expected taxonomic composition at several test sites into expected and observed trait compositions. The first three axes of a normalised PCA (Principal Components Analysis) performed on disturbance variables accounted for 42.7% of explained variability. The proportion of variance in distur- bance explained by the three types of trait-based metrics (overall observed/expected trait composition, trait-category profile difference and traits profile dissimilarity) ranged between 9% and 32%. Our pre- dictions made on the response of observed to expected trait categories for organic contamination were generally confirmed and demonstrated that disturbances resulted in a change in those traits conferring species resilience capacity and sensitivity to oxygen depletion, as well as a shift in the proportion of ani- mals with filter-feeding behaviour. Variations in observed to expected trait-category differences showed that even a small increase in organic contamination led to a significant change in the biological trait profile, as expected. By contrast, only two out of 11 trait category predictions were confirmed for hydro- logical disturbance. Finally, we found that 4/11 and 9/11 observed to expected trait differences showed a significant deviation with organic contamination and hydrological disturbance, respectively, whereas all 11 observed to expected trait differences responded to overall disturbance. These changes in trait profiles reflect changes in the performance of invertebrate communities to cope with disturbance, which potentially can alter ecosystem functioning (e.g., energy flow or chemical cycling). In conclusion, the integration of biological trait information in an AUSRIVAS type predictive model allowed the detection of a general disturbance gradient and particularly organic contamination, which indicates their value in addition to taxonomic-based assessment.